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Part:BBa_K539733:Design

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-24)

kivd (alpha-ketoisovalerate decarboxylase) with native rbs(BBa_B0034) and terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1673
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is an available coding sequence that can be placed at the end of the circuit. We also made an similar biobrick that the different rbs is included.


Source

It is cloned form Lactococcus lactis subsp, lactis KF147, by Zeng lab, NCTU-Formosa.


References